• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

APC15 Polyclonal antibody

APC15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20409-1-AP

Print datasheet

Synonyms

C11orf51, HSPC020, Anaphase-promoting complex subunit 15, ANAPC15

验证数据展示

  • WB analysis of HT-29 using 20409-1-AP

    WB analysis of HT-29 using 20409-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20409-1-AP

    IHC staining of human placenta using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of mouse adrenal gland using 20409-1-AP

    IHC staining of mouse adrenal gland using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of mouse lung using 20409-1-AP

    IHC staining of mouse lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of mouse spleen using 20409-1-AP

    IHC staining of mouse spleen using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of rat adrenal gland using 20409-1-AP

    IHC staining of rat adrenal gland using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of rat lung using 20409-1-AP

    IHC staining of rat lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included

经过测试的应用

Positive WB detected inHT-29 cells, HEK-293 cells
Positive IHC detected inhuman placenta tissue, human lung tissue, human pancreas tissue, mouse adrenal gland tissue, mouse lung tissue, mouse spleen tissue, rat adrenal gland tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANAPC15 Ready-To-Use IHC Kit. ANAPC15 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20409-1-AP targets APC15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen APC15 fusion protein Ag14237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 51
Synonyms C11orf51, HSPC020, Anaphase-promoting complex subunit 15, ANAPC15
Calculated Molecular Weight 121 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC005156
Gene Symbol APC15
Gene ID (NCBI) 25906
RRIDAB_10694279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APC15 antibody 20409-1-APDownload protocol
IHC protocol for APC15 antibody 20409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols