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C11orf54 Polyclonal antibody

C11orf54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23251-1-AP

Print datasheet

Synonyms

C11orf54, Ester hydrolase C11orf54, PTD012

验证数据展示

  • WB analysis of rat liver using 23251-1-AP

    WB analysis of rat liver using 23251-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 23251-1-AP (C11orf54 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 23251-1-AP

    IHC staining of mouse liver using 23251-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 23251-1-AP (C11orf54 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inrat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23251-1-AP is C11orf54 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Immunogen C11orf54 fusion protein Ag19731 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 54
Synonyms C11orf54, Ester hydrolase C11orf54, PTD012
Calculated Molecular Weight 315 aa, 35 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC007110
Gene Symbol C11orf54
Gene ID (NCBI) 28970
RRIDAB_2879239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H0W9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf54 antibody 23251-1-APDownload protocol
IHC protocol for C11orf54 antibody 23251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Commun Biol

C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A

Authors - Junyang Tan
View Article