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C11orf57 Polyclonal antibody

C11orf57 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21181-1-AP

Print datasheet

Synonyms

C11orf57

验证数据展示

  • IHC staining of human breast cancer using 21181-1-AP

    IHC staining of human breast cancer using 21181-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21181-1-AP (C11orf57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 21181-1-AP

    IHC staining of human breast cancer using 21181-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21181-1-AP (C11orf57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 21181-1-AP

    WB analysis of mouse brain using 21181-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21181-1-AP (C11orf57 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21181-1-AP targets C11orf57 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C11orf57 fusion protein Ag15471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 57
Synonyms C11orf57
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC030546
Gene Symbol C11orf57
Gene ID (NCBI) 55216
RRIDAB_2878825
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZUT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf57 has three isoforms with MW 30-34 kDa.

实验方案

Product Specific Protocols
WB protocol for C11orf57 antibody 21181-1-APDownload protocol
IHC protocol for C11orf57 antibody 21181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways

Authors - Min Huang
View Article