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C11orf70 Polyclonal antibody

C11orf70 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20780-1-AP

Print datasheet

Synonyms

CK070, Cilia- and flagella-associated protein 300, CFAP300

验证数据展示

  • WB analysis of mouse testis using 20780-1-AP

    WB analysis of mouse testis using 20780-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20780-1-AP (C11orf70 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20780-1-AP

    IHC staining of human testis using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 20780-1-AP

    IHC staining of human testis using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 20780-1-AP

    IF Staining of HeLa using 20780-1-AP

    Immunofluorescent analysis of Hela cells, using C11orf70 antibody 20780-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse testis tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20780-1-AP targets C11orf70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C11orf70 fusion protein Ag14793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 70
Synonyms CK070, Cilia- and flagella-associated protein 300, CFAP300
Calculated Molecular Weight 267 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC006128
Gene Symbol C11orf70
Gene ID (NCBI) 85016
RRIDAB_10755147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf70 antibody 20780-1-APDownload protocol
IHC protocol for C11orf70 antibody 20780-1-APDownload protocol
IF protocol for C11orf70 antibody 20780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols