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验证数据展示

  • WB analysis using 19892-1-AP

    WB analysis using 19892-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19892-1-AP (ASUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 19892-1-AP

    IHC staining of human cervical cancer using 19892-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with ASUN antibody included
  • IHC staining of human cervical cancer using 19892-1-AP

    IHC staining of human cervical cancer using 19892-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with ASUN antibody included
  • IP experiment of MCF-7 using 19892-1-AP

    IP experiment of MCF-7 using 19892-1-AP

    IP result of anti-ASUN (IP:19892-1-AP, 4ug; Detection:19892-1-AP 1:8000) with MCF-7 cells lysate 1400 ug.

  • IF Staining of MCF-7 using 19892-1-AP

    IF Staining of MCF-7 using 19892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASUN antibody (19892-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ASUN Polyclonal antibody

ASUN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19892-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

INTS13, NET48, Integrator complex subunit 13, Germ cell tumor 1, GCT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ASUN Ready-To-Use IHC Kit. ASUN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19892-1-AP targets ASUN in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP, ChIP
经测试反应性 human
文献引用反应性human
免疫原 ASUN fusion protein Ag13727 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 12 open reading frame 11
别名 INTS13, NET48, Integrator complex subunit 13, Germ cell tumor 1, GCT1
计算分子量 706 aa, 80 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC003081
基因名称 ASUN
Gene ID (NCBI) 55726
RRIDAB_10638473
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

实验方案

Product Specific Protocols
WB protocol for ASUN antibody 19892-1-APDownload protocol
IHC protocol for ASUN antibody 19892-1-APDownload protocol
IF protocol for ASUN antibody 19892-1-APDownload protocol
IP protocol for ASUN antibody 19892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
View Article
humanWB

Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda
View Article
humanChIP, IP, WB

Nat Commun

Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes.

Authors - Bernd Stadelmayer
View Article
humanWB

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye
View Article