ASUN Polyclonal antibody
ASUN Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ASUN Ready-To-Use IHC Kit. ASUN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19892-1-AP targets ASUN in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, ChIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ASUN fusion protein Ag13727 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromosome 12 open reading frame 11 |
| Synonyms | INTS13, NET48, Integrator complex subunit 13, Germ cell tumor 1, GCT1 |
| Calculated Molecular Weight | 706 aa, 80 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC003081 |
| Gene Symbol | ASUN |
| Gene ID (NCBI) | 55726 |
| RRID | AB_10638473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVM9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASUN antibody 19892-1-AP | Download protocol |
| IHC protocol for ASUN antibody 19892-1-AP | Download protocol |
| IP protocol for ASUN antibody 19892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
Nat Commun Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes. | ||
Int J Clin Exp Pathol Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration. |
