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C13orf1 Polyclonal antibody

C13orf1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26510-1-AP

Print datasheet

Synonyms

SPRYD7, SPRY7, SPRY domain-containing protein 7, CLLD6

验证数据展示

  • IF Staining of PC-12 using 26510-1-AP

    IF Staining of PC-12 using 26510-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using C13orf1 antibody (26510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis of mouse brain using 26510-1-AP

    WB analysis of mouse brain using 26510-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26510-1-AP (C13orf1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 26510-1-AP

    IHC staining of human lymphoma using 26510-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26510-1-AP (C13orf1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 26510-1-AP

    IHC staining of human lymphoma using 26510-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26510-1-AP (C13orf1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26510-1-AP targets C13orf1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C13orf1 fusion protein Ag23487 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 13 open reading frame 1
Synonyms SPRYD7, SPRY7, SPRY domain-containing protein 7, CLLD6
Observed Molecular Weight 17 kDa, 20 kDa
GenBank Accession NumberBC022519
Gene Symbol C13orf1
Gene ID (NCBI) 57213
RRIDAB_2880536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5W111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C13orf1, also named as SPRYD7 and CLLD6, is involved in hematopoiesis via NOTCH signaling. C13orf1 plays an important role in the pathogenesis of B-CLL. There're some isoforms of C13orf1 with MW 15-17 kDa and 19-22 kDa.3

实验方案

Product Specific Protocols
WB protocol for C13orf1 antibody 26510-1-APDownload protocol
IHC protocol for C13orf1 antibody 26510-1-APDownload protocol
IF protocol for C13orf1 antibody 26510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols