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C14orf126 Polyclonal antibody

C14orf126 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23923-1-AP

Print datasheet

Synonyms

EC:3.1.1.96, D-tyrosyl-tRNA(Tyr) deacylase 2, DTD2, D-aminoacyl-tRNA deacylase 2, ATD

验证数据展示

  • WB analysis using 23923-1-AP

    WB analysis using 23923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 23923-1-AP

    WB analysis using 23923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 23923-1-AP

    IF Staining of A549 using 23923-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C14orf126 antibody (23923-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, mouse cerebellum tissue, HepG2 cells, mouse lung tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23923-1-AP targets C14orf126 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C14orf126 fusion protein Ag21053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 126
Synonyms EC:3.1.1.96, D-tyrosyl-tRNA(Tyr) deacylase 2, DTD2, D-aminoacyl-tRNA deacylase 2, ATD
Calculated Molecular Weight 168 aa, 19 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC010618
Gene Symbol C14orf126
Gene ID (NCBI) 112487
RRIDAB_3669426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96FN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C14orf126, also named D-aminoacyl-tRNA deacylase 2 (DTD2), can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Plants are unique in possessing two distinct chiral proofreading systems, D-aminoacyl-tRNA deacylase1 (DTD1) and DTD2, of bacterial and archaeal origins, respectively.

实验方案

Product Specific Protocols
WB protocol for C14orf126 antibody 23923-1-APDownload protocol
IF protocol for C14orf126 antibody 23923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols