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C14orf126 Polyclonal antibody

C14orf126 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23923-1-AP

Print datasheet

Synonyms

EC:3.1.1.96, D-tyrosyl-tRNA(Tyr) deacylase 2, DTD2, D-aminoacyl-tRNA deacylase 2, ATD

验证数据展示

  • WB analysis using 23923-1-AP

    WB analysis using 23923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 23923-1-AP

    WB analysis using 23923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23923-1-AP (C14orf126 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 23923-1-AP

    IF Staining of A549 using 23923-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C14orf126 antibody (23923-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, mouse cerebellum tissue, HepG2 cells, mouse lung tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23923-1-AP targets C14orf126 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C14orf126 fusion protein Ag21053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 126
Synonyms EC:3.1.1.96, D-tyrosyl-tRNA(Tyr) deacylase 2, DTD2, D-aminoacyl-tRNA deacylase 2, ATD
Calculated Molecular Weight 168 aa, 19 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC010618
Gene Symbol C14orf126
Gene ID (NCBI) 112487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96FN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C14orf126, also named D-aminoacyl-tRNA deacylase 2 (DTD2), can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Plants are unique in possessing two distinct chiral proofreading systems, D-aminoacyl-tRNA deacylase1 (DTD1) and DTD2, of bacterial and archaeal origins, respectively.

实验方案

Product Specific Protocols
WB protocol for C14orf126 antibody 23923-1-APDownload protocol
IF protocol for C14orf126 antibody 23923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols