验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, human kidney tissue, HepG2 cells, L02 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HEK-293 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
20771-1-AP targets VIPAS39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | VIPAS39 fusion protein Ag14762 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 14 open reading frame 133 |
| 别名 | C14orf133, hSPE 39, Protein spe 39 homolog, VIPAR, VIPAS39, VPS33B interacting protein |
| 计算分子量 | 493 aa, 57 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC015054 |
| 基因名称 | VIPAS39 |
| Gene ID (NCBI) | 63894 |
| RRID | AB_10695764 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9C1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VIPAS39 antibody 20771-1-AP | Download protocol |
| IHC protocol for VIPAS39 antibody 20771-1-AP | Download protocol |
| IF protocol for VIPAS39 antibody 20771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virus Genes Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7. | ||
Adv Sci (Weinh) Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase |
