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C14orf138 Polyclonal antibody

C14orf138 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24722-1-AP

Print datasheet

Synonyms

C14orf138

验证数据展示

  • WB analysis of PC-3 using 24722-1-AP

    WB analysis of PC-3 using 24722-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24722-1-AP

    IHC staining of mouse testis using 24722-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, HepG2 cells, human testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24722-1-AP targets C14orf138 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C14orf138 fusion protein Ag20154 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 138
Synonyms C14orf138
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC105118
Gene Symbol C14orf138
Gene ID (NCBI) 79609
RRIDAB_2879689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H867
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.

实验方案

Product Specific Protocols
WB protocol for C14orf138 antibody 24722-1-APDownload protocol
IHC protocol for C14orf138 antibody 24722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols