• Featured Product
  • KD/KO Validated

SLIRP Polyclonal antibody

SLIRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26006-1-AP

Print datasheet

Synonyms

SRA stem-loop-interacting RNA-binding protein, mitochondrial, PD04872, DC50, DC23, C14orf156



经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, MDA-MB-453s cells
Positive IHC detected inhuman ovary cancer tissue, human kidney tissue, human stomach cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26006-1-AP targets SLIRP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SLIRP fusion protein Ag22945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 156
Synonyms SRA stem-loop-interacting RNA-binding protein, mitochondrial, PD04872, DC50, DC23, C14orf156
Calculated Molecular Weight 109 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC017895
Gene Symbol SLIRP
Gene ID (NCBI) 81892
RRIDAB_2880332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLIRP, also named as C14orf156, DC23, DC50 and PD04872. It is an RNA-binding protein on steroid receptor RNA activator (SRA), and its expression is ubiquitous in human normal tissues. SLIRP can affect mitochondrial mRNA transcription and energy conversion. In addition, the loss or weakening of SLIRP destroys part of the structure of mitochondria, affects mitochondria energy metabolism, and increases sperm or cell oxidative stress damage (PMID: 33150185). SLIRP has 2 isoforms with the molecular mass of 12 kDa.

实验方案

Product Specific Protocols
WB protocol for SLIRP antibody 26006-1-APDownload protocol
IHC protocol for SLIRP antibody 26006-1-APDownload protocol
IF protocol for SLIRP antibody 26006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,CoIP

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
  • KD Validated
humanWB

Methods Mol Biol

Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA

Authors - Kah Ying Ng
humanWB

J Transl Med

A traditional gynecological medicine inhibits ovarian cancer progression and eliminates cancer stem cells via the LRPPRC-OXPHOS axis

Authors - Ruibin Jiang
humanWB

Int J Mol Sci

Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells

Authors - Mariia V Baleva
humanWB

Cell Death Differ

C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer

Authors - Hui Wang