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C14orf2 Polyclonal antibody

C14orf2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14704-1-AP

Print datasheet

Synonyms

C14orf2, MP68, PLPM

验证数据展示

  • WB analysis of Jurkat using 14704-1-AP

    WB analysis of Jurkat using 14704-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14704-1-AP (C14orf2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14704-1-AP

    IP experiment of Jurkat using 14704-1-AP

    IP result of anti-C14orf2 (IP:14704-1-AP, 4ug; Detection:14704-1-AP 1:300) with Jurkat cells lysate 3360ug.

  • IHC staining of human heart using 14704-1-AP

    IHC staining of human heart using 14704-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14704-1-AP (C14orf2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 14704-1-AP

    IHC staining of human heart using 14704-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14704-1-AP (C14orf2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, human brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14704-1-AP targets C14orf2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen C14orf2 fusion protein Ag6386 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 2
Synonyms C14orf2, MP68, PLPM
Calculated Molecular Weight 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC001944
Gene Symbol C14orf2
Gene ID (NCBI) 9556
RRIDAB_2228192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C14orf2 antibody 14704-1-APDownload protocol
IHC protocol for C14orf2 antibody 14704-1-APDownload protocol
IP protocol for C14orf2 antibody 14704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols