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C14orf28 Polyclonal antibody

C14orf28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26130-1-AP

Print datasheet

Synonyms

c14_5270, C14orf28, DRIP1

验证数据展示

  • WB analysis of HeLa using 26130-1-AP

    WB analysis of HeLa using 26130-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26130-1-AP (C14orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26130-1-AP

    IP experiment of HeLa using 26130-1-AP

    IP result of anti-C14orf28 (IP:26130-1-AP, 4ug; Detection:26130-1-AP 1:300) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 26130-1-AP

    IF Staining of HeLa using 26130-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26130-1-AP (C14orf28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26130-1-AP targets C14orf28 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen C14orf28 fusion protein Ag23441 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 28
Synonyms c14_5270, C14orf28, DRIP1
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC105030
Gene Symbol C14orf28
Gene ID (NCBI) 122525
RRIDAB_2880393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4W4Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C14orf28 antibody 26130-1-APDownload protocol
IF protocol for C14orf28 antibody 26130-1-APDownload protocol
IP protocol for C14orf28 antibody 26130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols