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验证数据展示

  • WB analysis of Jurkat using 22664-1-AP

    WB analysis of Jurkat using 22664-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22664-1-AP

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • IHC staining of human skeletal muscle using 22664-1-AP

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 22664-1-AP

    IF Staining of MCF-7 using 22664-1-AP

    Immunofluorescent analysis of MCF-7 cells using 22664-1-AP (C14orf39 antibody) at dilution of 1:50.

  • IF Staining of mouse testis using 22664-1-AP

    IF Staining of mouse testis using 22664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C14orf39 antibody (22664-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

C14orf39 Polyclonal antibody

C14orf39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22664-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

SIX6OS1, Six6 opposite strand transcript 1, Protein SIX6OS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse colon tissue, SKOV-3 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22664-1-AP targets C14orf39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 C14orf39 fusion protein Ag18589 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 39
别名 SIX6OS1, Six6 opposite strand transcript 1, Protein SIX6OS1
计算分子量 587 aa, 68 kDa
观测分子量 50 kDa
GenBank蛋白编号BC125070
基因名称 C14orf39
Gene ID (NCBI) 317761
RRIDAB_2879146
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1H7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C14orf39 antibody 22664-1-APDownload protocol
IHC protocol for C14orf39 antibody 22664-1-APDownload protocol
IF protocol for C14orf39 antibody 22664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.

Authors - Laura Gómez-H
  • KO Validated
View Article