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C14orf94 Polyclonal antibody

C14orf94 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20115-1-AP

Print datasheet

Synonyms

C14orf94, HAUS4

验证数据展示

  • WB analysis of PC-3 using 20115-1-AP

    WB analysis of PC-3 using 20115-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20115-1-AP (C14orf94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 20115-1-AP

    IHC staining of human lung using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 20115-1-AP

    IHC staining of human lung using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inPC-3 cells, mouse skin tissue
Positive IHC detected inhuman lung tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20115-1-AP is C14orf94 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C14orf94 fusion protein Ag13986 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 94
Synonyms C14orf94, HAUS4
Calculated Molecular Weight 363 aa, 42 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC001916
Gene Symbol C14orf94
Gene ID (NCBI) 54930
RRIDAB_10638430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6D7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

实验方案

Product Specific Protocols
WB protocol for C14orf94 antibody 20115-1-APDownload protocol
IHC protocol for C14orf94 antibody 20115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols