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验证数据展示

  • WB analysis of PC-3 using 20115-1-AP

    WB analysis of PC-3 using 20115-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20115-1-AP (C14orf94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 20115-1-AP

    IHC staining of human lung using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 20115-1-AP

    IHC staining of human lung using 20115-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 40x lens).

C14orf94 Polyclonal antibody

C14orf94 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20115-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C14orf94, HAUS4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse skin tissue
Positive IHC detected inhuman lung tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20115-1-AP targets C14orf94 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C14orf94 fusion protein Ag13986 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 94
别名 C14orf94, HAUS4
计算分子量 363 aa, 42 kDa
观测分子量 28 kDa
GenBank蛋白编号BC001916
基因名称 C14orf94
Gene ID (NCBI) 54930
RRIDAB_10638430
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6D7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

实验方案

Product Specific Protocols
WB protocol for C14orf94 antibody 20115-1-APDownload protocol
IHC protocol for C14orf94 antibody 20115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols