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C15orf58 Polyclonal antibody

C15orf58 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25447-1-AP

Print datasheet

Synonyms

C15orf58, UPF0580 protein C15orf58

验证数据展示

  • WB analysis of PC-3 using 25447-1-AP

    WB analysis of PC-3 using 25447-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 25447-1-AP

    IF Staining of PC-3 using 25447-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25447-1-AP (C15orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, mouse brain tissue
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25447-1-AP is C15orf58 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C15orf58 fusion protein Ag20215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 15 open reading frame 58
Synonyms C15orf58, UPF0580 protein C15orf58
Calculated Molecular Weight 385 aa, 42 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC127821
Gene Symbol C15orf58
Gene ID (NCBI) 390637
RRIDAB_2880085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZNW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C15orf58 antibody 25447-1-APDownload protocol
IF protocol for C15orf58 antibody 25447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols