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验证数据展示

  • WB analysis using 20808-1-AP

    WB analysis using 20808-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20808-1-AP (C16orf14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 20808-1-AP

    IHC staining of mouse heart using 20808-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20808-1-AP (C16orf14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 20808-1-AP

    IHC staining of mouse heart using 20808-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20808-1-AP (C16orf14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 20808-1-AP

    IF Staining of HepG2 using 20808-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C16orf14 antibody (20808-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C16orf14 Polyclonal antibody

C16orf14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20808-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Protein FAM195A, MCRIP2, MAPK regulated corepressor interacting protein 2, FAM195A, c349E10.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse skin tissue, K-562 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20808-1-AP targets C16orf14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 C16orf14 fusion protein Ag14754 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 14
别名 Protein FAM195A, MCRIP2, MAPK regulated corepressor interacting protein 2, FAM195A, c349E10.1
计算分子量 160 aa, 18 kDa
观测分子量 8 kDa, 18 kDa
GenBank蛋白编号BC007346
基因名称 C16orf14
Gene ID (NCBI) 84331
RRIDAB_10700013
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUT9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C16orf14 antibody 20808-1-APDownload protocol
IHC protocol for C16orf14 antibody 20808-1-APDownload protocol
IF protocol for C16orf14 antibody 20808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Biomolecules

Comprehensive Protein Interactome Analysis of a Key RNA Helicase: Detection of Novel Stress Granule Proteins.

Authors - Rebecca Bish
View Article
humanWB

bioRxiv

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Authors - Sihang Zhou
View Article