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C16orf14 Polyclonal antibody

C16orf14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20808-1-AP

Print datasheet

Synonyms

Protein FAM195A, MCRIP2, MAPK regulated corepressor interacting protein 2, FAM195A, c349E10.1

验证数据展示

  • WB analysis using 20808-1-AP

    WB analysis using 20808-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20808-1-AP (C16orf14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20808-1-AP

    IF Staining of HeLa using 20808-1-AP

    Immunofluorescent analysis of Hela cells, using C16orf14 antibody 20808-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of mouse heart using 20808-1-AP

    IHC staining of mouse heart using 20808-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20808-1-AP (C16orf14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 20808-1-AP

    IHC staining of mouse heart using 20808-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20808-1-AP (C16orf14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse skin tissue, K-562 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20808-1-AP targets C16orf14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C16orf14 fusion protein Ag14754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 16 open reading frame 14
Synonyms Protein FAM195A, MCRIP2, MAPK regulated corepressor interacting protein 2, FAM195A, c349E10.1
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 8 kDa, 18 kDa
GenBank Accession NumberBC007346
Gene Symbol C16orf14
Gene ID (NCBI) 84331
RRIDAB_10700013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C16orf14 antibody 20808-1-APDownload protocol
IHC protocol for C16orf14 antibody 20808-1-APDownload protocol
IF protocol for C16orf14 antibody 20808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Biomolecules

Comprehensive Protein Interactome Analysis of a Key RNA Helicase: Detection of Novel Stress Granule Proteins.

Authors - Rebecca Bish
View Article
humanWB

bioRxiv

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Authors - Sihang Zhou
View Article