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验证数据展示

  • WB analysis using 20354-1-AP

    WB analysis using 20354-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20354-1-AP (PA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PA1 Polyclonal antibody

PA1 Polyclonal Antibody for WB, ELISA
Cat No. 20354-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C16orf53, PAXIP1 associated protein 1, PAXIP1 Associated Glutamate Rich Protein 1, PAGR1, Glutamate-rich coactivator interacting with SRC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20354-1-AP targets PA1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PA1 fusion protein Ag14198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 53
别名 C16orf53, PAXIP1 associated protein 1, PAXIP1 Associated Glutamate Rich Protein 1, PAGR1, Glutamate-rich coactivator interacting with SRC1
计算分子量 254 aa, 28 kDa
观测分子量40-42 kDa
GenBank蛋白编号BC003640
基因名称 PA1
Gene ID (NCBI) 79447
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAGR1 (PAXIP1 Associated Glutamate Rich Protein 1), also known as PA1 or C16orf53, the PA1 protein forms a complex with the histone H3K4 methyltransferases MLL3 and MLL4 and interacts with PAXIP1 (PAX Interacting Protein 1). PA1 is associated with the DNA damage response, is involved in early embryonic development, and may play a key role in male reproduction. PA1 has estrogen receptor binding activity and is involved in the endogenous positive regulation of the intracellular estrogen receptor signaling pathway.

实验方案

Product Specific Protocols
WB protocol for PA1 antibody 20354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols