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VPS35L Polyclonal antibody

VPS35L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26923-1-AP

Print datasheet

Synonyms

UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62

验证数据展示

  • WB analysis using 26923-1-AP

    WB analysis using 26923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26923-1-AP (VPS35L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, MOLT-4 cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen VPS35L fusion protein Ag25620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 16 open reading frame 62
Synonyms UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62
Observed Molecular Weight98 kDa
GenBank Accession NumberBC058845
Gene Symbol C16orf62
Gene ID (NCBI) 57020
RRIDAB_3669574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3J2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.

实验方案

Product Specific Protocols
WB protocol for VPS35L antibody 26923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols