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VPS35L Polyclonal antibody

VPS35L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26923-1-AP

Print datasheet

Synonyms

UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62

验证数据展示

  • WB analysis using 26923-1-AP

    WB analysis using 26923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26923-1-AP (VPS35L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, MOLT-4 cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen VPS35L fusion protein Ag25620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 16 open reading frame 62
Synonyms UPF0505 protein C16orf62, RTSC3, Esophageal cancer-associated protein, EC97, C16orf62
Observed Molecular Weight98 kDa
GenBank Accession NumberBC058845
Gene Symbol C16orf62
Gene ID (NCBI) 57020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3J2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.

实验方案

Product Specific Protocols
WB protocol for VPS35L antibody 26923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols