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验证数据展示

  • WB analysis of HepG2 using 23337-1-AP

    WB analysis of HepG2 using 23337-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 23337-1-AP

    IP experiment of HepG2 using 23337-1-AP

    IP result of anti-C16orf84 (IP:23337-1-AP, 4ug; Detection:23337-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • IHC staining of human testis using 23337-1-AP

    IHC staining of human testis using 23337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 23337-1-AP

    IHC staining of human kidney using 23337-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 23337-1-AP

    IF Staining of HEK-293 using 23337-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C16orf84 antibody (23337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C16orf84 Polyclonal antibody

C16orf84 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 23337-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

UPF0432, NCS2, Cytosolic thiouridylase subunit 2, Cytoplasmic tRNA 2-thiolation protein 2, CTU2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23337-1-AP targets C16orf84 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 C16orf84 fusion protein Ag18428 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 84
别名 UPF0432, NCS2, Cytosolic thiouridylase subunit 2, Cytoplasmic tRNA 2-thiolation protein 2, CTU2
计算分子量 515 aa, 56 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC125269
基因名称 C16orf84
Gene ID (NCBI) 348180
RRIDAB_2879257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2VPK5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.

实验方案

Product Specific Protocols
WB protocol for C16orf84 antibody 23337-1-APDownload protocol
IHC protocol for C16orf84 antibody 23337-1-APDownload protocol
IF protocol for C16orf84 antibody 23337-1-APDownload protocol
IP protocol for C16orf84 antibody 23337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols