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  • Featured Product
  • KD/KO Validated

C17orf28 Polyclonal antibody

C17orf28 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21174-1-AP

Print datasheet

Synonyms

Protein hid-1 homolog, Protein HID1, HID1 domain-containing protein, HID1, Down-regulated in multiple cancers 1

验证数据展示

  • WB analysis of fetal human brain using 21174-1-AP

    WB analysis of fetal human brain using 21174-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21174-1-AP (C17orf28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21174-1-AP

    IP experiment of mouse brain using 21174-1-AP

    IP result of anti-C17orf28 (IP:21174-1-AP, 3ug; Detection:21174-1-AP 1:500) with mouse brain tissue lysate 10000ug.

  • IF Staining of HepG2 using 21174-1-AP

    IF Staining of HepG2 using 21174-1-AP

    Immunofluorescent analysis of HepG2 cells, using C17orf28 antibody 21174-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human stomach cancer using 21174-1-AP

    IHC staining of human stomach cancer using 21174-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21174-1-AP (C17orf28 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21174-1-AP targets C17orf28 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen C17orf28 fusion protein Ag15427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 17 open reading frame 28
Synonyms Protein hid-1 homolog, Protein HID1, HID1 domain-containing protein, HID1, Down-regulated in multiple cancers 1
Calculated Molecular Weight 788 aa, 89 kDa
Observed Molecular Weight 60 kDa, 89 kDa
GenBank Accession NumberBC035372
Gene Symbol C17orf28
Gene ID (NCBI) 283987
RRIDAB_10733124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C17orf28 antibody 21174-1-APDownload protocol
IHC protocol for C17orf28 antibody 21174-1-APDownload protocol
IF protocol for C17orf28 antibody 21174-1-APDownload protocol
IP protocol for C17orf28 antibody 21174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Elife

HID-1 is required for homotypic fusion of immature secretory granules during maturation.

Authors - Wen Du
  • KO Validated
View Article
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li
View Article