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  • KD/KO Validated

C17orf28 Polyclonal antibody

C17orf28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21174-1-AP

Print datasheet

Synonyms

C17orf28, DMC1, HID1



经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21174-1-AP is C17orf28 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen C17orf28 fusion protein Ag15427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 17 open reading frame 28
Synonyms C17orf28, DMC1, HID1
Calculated Molecular Weight 788 aa, 89 kDa
Observed Molecular Weight 60 kDa, 89 kDa
GenBank Accession NumberBC035372
Gene Symbol C17orf28
Gene ID (NCBI) 283987
RRIDAB_10733124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C17orf28 antibody 21174-1-APDownload protocol
IF protocol for C17orf28 antibody 21174-1-APDownload protocol
IP protocol for C17orf28 antibody 21174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Elife

HID-1 is required for homotypic fusion of immature secretory granules during maturation.

Authors - Wen Du
  • KO Validated
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li