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C17orf57 Polyclonal antibody

C17orf57 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27603-1-AP

Print datasheet

Synonyms

EFCAB13, EF-hand calcium-binding domain-containing protein 13

验证数据展示

  • IHC staining of mouse cerebellum using 27603-1-AP

    IHC staining of mouse cerebellum using 27603-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27603-1-AP (C17orf57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27603-1-AP

    IHC staining of mouse cerebellum using 27603-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27603-1-AP (C17orf57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 27603-1-AP

    IF Staining of mouse cerebellum using 27603-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using C17orf57 antibody (27603-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27603-1-AP targets C17orf57 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C17orf57 fusion protein Ag24015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 17 open reading frame 57
Synonyms EFCAB13, EF-hand calcium-binding domain-containing protein 13
Observed Molecular Weight110 kDa
GenBank Accession NumberBC036407
Gene Symbol C17orf57
Gene ID (NCBI) 124989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for C17orf57 antibody 27603-1-APDownload protocol
IF protocol for C17orf57 antibody 27603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols