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验证数据展示

  • WB analysis using 10162-1-AP

    WB analysis using 10162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10162-1-AP (C17orf81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10162-1-AP

    WB analysis of HEK-293 using 10162-1-AP

    WB result of C17orf81 antibody (10162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf81 transfected HEK-293 cells.

  • IHC staining of mouse heart using 10162-1-AP

    IHC staining of mouse heart using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10162-1-AP

    IHC staining of mouse brain using 10162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

C17orf81 Polyclonal antibody

C17orf81 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10162-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MSTP071, MST071, HSPC002, ELP5, Elongator complex protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10162-1-AP targets C17orf81 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C17orf81 fusion protein Ag0194 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 17 open reading frame 81
别名 MSTP071, MST071, HSPC002, ELP5, Elongator complex protein 5
计算分子量 35 kDa
观测分子量 37 and 45 kDa
GenBank蛋白编号BC002762
基因名称 C17orf81
Gene ID (NCBI) 23587
RRIDAB_2274975
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TE02
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELP5, also named as C17orf81, DERP6 and S-phase 2 protein, acts as subunit of the RNA polymerase II elongator complex. It's involved in TP53-mediated transcriptional regulation. ELP5 has some isoforms with MW 20 kDa, 30 kDa and 34-37 kDa.

实验方案

Product Specific Protocols
WB protocol for C17orf81 antibody 10162-1-APDownload protocol
IHC protocol for C17orf81 antibody 10162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols