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RMC1 Polyclonal antibody

RMC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20111-1-AP

Print datasheet

Synonyms

MIC1, C18orf8, Mic-1, Mic 1, HsT2591

验证数据展示

  • WB analysis of human brain using 20111-1-AP

    WB analysis of human brain using 20111-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20111-1-AP (RMC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 20111-1-AP

    IHC staining of human colon cancer using 20111-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20111-1-AP (RMC1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon cancer using 20111-1-AP

    IHC staining of human colon cancer using 20111-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20111-1-AP (C18orf8 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of MCF-7 using 20111-1-AP

    IP experiment of MCF-7 using 20111-1-AP

    IP result of anti-RMC1 (IP:20111-1-AP, 4ug; Detection:20111-1-AP 1:600) with MCF-7 cells lysate 1120 ug.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20111-1-AP targets RMC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RMC1 fusion protein Ag13904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 18 open reading frame 8
Synonyms MIC1, C18orf8, Mic-1, Mic 1, HsT2591
Calculated Molecular Weight 657 aa, 75 kDa
Observed Molecular Weight 75-80 kDa, 66 kDa
GenBank Accession NumberBC002950
Gene Symbol RMC1
Gene ID (NCBI) 29919
RRIDAB_10646439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RMC1 antibody 20111-1-APDownload protocol
IHC protocol for RMC1 antibody 20111-1-APDownload protocol
IP protocol for RMC1 antibody 20111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols