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C19orf36 Polyclonal antibody

C19orf36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25650-1-AP

Print datasheet

Synonyms

IZUMO4, Izumo sperm-egg fusion protein 4

验证数据展示

  • WB analysis of mouse testis using 25650-1-AP

    WB analysis of mouse testis using 25650-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25650-1-AP

    IHC staining of mouse testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse testis using 25650-1-AP

    IHC staining of mouse testis using 25650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25650-1-AP

    IF Staining of HepG2 using 25650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C19orf36 antibody (25650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25650-1-AP targets C19orf36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitybovine
Immunogen C19orf36 fusion protein Ag20434 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 36
Synonyms IZUMO4, Izumo sperm-egg fusion protein 4
Calculated Molecular Weight 232 aa, 27 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC014609
Gene Symbol C19orf36
Gene ID (NCBI) 113177
RRIDAB_2880175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1ZYL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IZUMO4, also named as C19orf36, is sperm 22 kDa protein c113. It has 4 isoforms with MW 17-27 kDa.

实验方案

Product Specific Protocols
WB protocol for C19orf36 antibody 25650-1-APDownload protocol
IHC protocol for C19orf36 antibody 25650-1-APDownload protocol
IF protocol for C19orf36 antibody 25650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineIF,WB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article