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C19orf50 Polyclonal antibody

C19orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 20492-1-AP

Print datasheet

Synonyms

KXD1, UPF0459 protein C19orf50, MSTP096, MST096, KxDL motif-containing protein 1

验证数据展示

  • WB analysis using 20492-1-AP

    WB analysis using 20492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20492-1-AP (C19orf50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 20492-1-AP

    IHC staining of mouse cerebellum using 20492-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 20492-1-AP

    IF Staining of A549 using 20492-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C19orf50 antibody (20492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, Calu-3 cells, NCI-H1299 cells, human placenta tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20492-1-AP targets C19orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C19orf50 fusion protein Ag13743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 50
Synonyms KXD1, UPF0459 protein C19orf50, MSTP096, MST096, KxDL motif-containing protein 1
Calculated Molecular Weight 176 aa, 20 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC001080
Gene Symbol C19orf50
Gene ID (NCBI) 79036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQD3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C19orf50, also known as KXD1 (KxDL motif-containing protein 1), a human uncharacterized coiled-coil protein, is a BLOS1-interacting protein. C19orf50 has been shown recently to interact with BLOC-1 subunits and may play a role in lysosome movement and localization at the cell periphery. C19orf50 was evaluated in NSCLC and LUAD (PMID: 33936200, 22554196).

实验方案

Product Specific Protocols
WB protocol for C19orf50 antibody 20492-1-APDownload protocol
IHC protocol for C19orf50 antibody 20492-1-APDownload protocol
IF protocol for C19orf50 antibody 20492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols