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验证数据展示

  • WB analysis using 20492-1-AP

    WB analysis using 20492-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20492-1-AP (C19orf50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 20492-1-AP

    IHC staining of mouse cerebellum using 20492-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20492-1-AP (C19orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 20492-1-AP

    IF Staining of A549 using 20492-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C19orf50 antibody (20492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C19orf50 Polyclonal antibody

C19orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20492-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

KXD1, UPF0459 protein C19orf50, MSTP096, MST096, KxDL motif-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Calu-3 cells, NCI-H1299 cells, human placenta tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20492-1-AP targets C19orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C19orf50 fusion protein Ag13743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 50
别名 KXD1, UPF0459 protein C19orf50, MSTP096, MST096, KxDL motif-containing protein 1
计算分子量 176 aa, 20 kDa
观测分子量28 kDa
GenBank蛋白编号BC001080
基因名称 C19orf50
Gene ID (NCBI) 79036
RRIDAB_3669335
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQD3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C19orf50, also known as KXD1 (KxDL motif-containing protein 1), a human uncharacterized coiled-coil protein, is a BLOS1-interacting protein. C19orf50 has been shown recently to interact with BLOC-1 subunits and may play a role in lysosome movement and localization at the cell periphery. C19orf50 was evaluated in NSCLC and LUAD (PMID: 33936200, 22554196).

实验方案

Product Specific Protocols
WB protocol for C19orf50 antibody 20492-1-APDownload protocol
IHC protocol for C19orf50 antibody 20492-1-APDownload protocol
IF protocol for C19orf50 antibody 20492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols