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验证数据展示

  • WB analysis of L02 using 26299-1-AP

    WB analysis of L02 using 26299-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 26299-1-AP (C19orf54 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26299-1-AP

    IHC staining of human liver using 26299-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26299-1-AP (C19orf54 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26299-1-AP

    IHC staining of human liver using 26299-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26299-1-AP (C19orf54 Antibody) at dilution of 1:200 (under 40x lens).

C19orf54 Polyclonal antibody

C19orf54 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26299-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C19orf54, UPF0692 protein C19orf54

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, A549 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26299-1-AP targets C19orf54 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 C19orf54 fusion protein Ag23652 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 54
别名 C19orf54, UPF0692 protein C19orf54
观测分子量 32-36 kDa
GenBank蛋白编号BC090894
基因名称 C19orf54
Gene ID (NCBI) 284325
RRIDAB_2880469
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5BKX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf54 antibody 26299-1-APDownload protocol
IHC protocol for C19orf54 antibody 26299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols