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  • Featured Product
  • KD/KO Validated

C19orf57 Polyclonal antibody

C19orf57 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25050-1-AP

Print datasheet

Synonyms

Pre-T/NK cell-associated protein 3B3, BRME1, Break repair meiotic recombinase recruitment factor 1

验证数据展示

  • WB analysis using 25050-1-AP

    WB analysis using 25050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25050-1-AP

    IHC staining of mouse testis using 25050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25050-1-AP (C19orf57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 25050-1-AP

    WB analysis of Jurkat using 25050-1-AP

    WB result of C19orf57 antibody (25050-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf57 transfected Jurkat cells.

  • WB analysis of PC-3 using 25050-1-AP

    WB analysis of PC-3 using 25050-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25050-1-AP

    IF Staining of A431 using 25050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf57 antibody (25050-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, PC-3 cells, A431 cells, A2780 cells, MOLT-4 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25050-1-AP targets C19orf57 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C19orf57 fusion protein Ag21745 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 57
Synonyms Pre-T/NK cell-associated protein 3B3, BRME1, Break repair meiotic recombinase recruitment factor 1
Calculated Molecular Weight 668 aa, 70 kDa
Observed Molecular Weight 60-100 kDa
GenBank Accession NumberBC119719
Gene Symbol C19orf57
Gene ID (NCBI) 79173
RRIDAB_2879871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VDD7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf57 antibody 25050-1-APDownload protocol
IHC protocol for C19orf57 antibody 25050-1-APDownload protocol
IF protocol for C19orf57 antibody 25050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols