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MCEMP1 Polyclonal antibody

MCEMP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25700-1-AP

Print datasheet

Synonyms

C19orf59, MCEMP1

验证数据展示

  • WB analysis using 25700-1-AP

    WB analysis using 25700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25700-1-AP (C19orf59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 25700-1-AP

    IHC staining of human lung using 25700-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25700-1-AP (C19orf59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 25700-1-AP

    IHC staining of mouse lung using 25700-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25700-1-AP (C19orf59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inTHP-1 cells, mouse liver tissue
Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25700-1-AP targets MCEMP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MCEMP1 fusion protein Ag22616 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 59
Synonyms C19orf59, MCEMP1
Observed Molecular Weight22 kDa
GenBank Accession NumberBC104798
Gene Symbol MCEMP1
Gene ID (NCBI) 199675
RRIDAB_2918093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IX19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. An evidently increased expression of mast cell expression membrane protein 1 (MCEMP1) has been observed in stroke patients and its expression independently improved the discrimination of 1-month outcome, suggesting the functionality of peripheral blood expression of MCEMP1 as a biomarker for stroke prognosis . (PMID: 30883005, PMID: 31104315, PMID: 15953541)

实验方案

Product Specific Protocols
WB protocol for MCEMP1 antibody 25700-1-APDownload protocol
IHC protocol for MCEMP1 antibody 25700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PeerJ

Identification of a novel immune infiltration-related gene signature, MCEMP1, for coronary artery disease

Authors - Wei Ye
View Article