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C19orf62 Polyclonal antibody

C19orf62 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27926-1-AP

Print datasheet

Synonyms

BABAM1, C19orf62, HSPC142, MERIT40, NBA1

验证数据展示

  • WB analysis using 27926-1-AP

    WB analysis using 27926-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27926-1-AP (C19orf62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27926-1-AP

    WB analysis of HepG2 using 27926-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27926-1-AP (C19orf62 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27926-1-AP

    IHC staining of human breast cancer using 27926-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27926-1-AP (C19orf62 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27926-1-AP

    IHC staining of human breast cancer using 27926-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27926-1-AP (C19orf62 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27926-1-AP targets C19orf62 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen C19orf62 fusion protein Ag27459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 19 open reading frame 62
Synonyms BABAM1, C19orf62, HSPC142, MERIT40, NBA1
Calculated Molecular Weight 329 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC000788
Gene Symbol C19orf62
Gene ID (NCBI) 29086
RRIDAB_2881010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C19orf62 antibody 27926-1-APDownload protocol
IHC protocol for C19orf62 antibody 27926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols