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验证数据展示

  • WB analysis using 15757-1-AP

    WB analysis using 15757-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15757-1-AP (C1QL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 15757-1-AP

    IHC staining of mouse cerebellum using 15757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 15757-1-AP

    IHC staining of mouse cerebellum using 15757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 15757-1-AP

    IF Staining of mouse cerebellum using 15757-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using C1QL1 antibody (15757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C1QL1 Polyclonal antibody

C1QL1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 15757-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CRF, Complement component 1 Q subcomponent-like 1, C1QRF, C1q-related factor, C1q/TNF-related protein 14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15757-1-AP targets C1QL1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen C1QL1 fusion protein Ag8348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 1, q subcomponent-like 1
Synonyms CRF, Complement component 1 Q subcomponent-like 1, C1QRF, C1q-related factor, C1q/TNF-related protein 14
Calculated Molecular Weight 258 aa, 26 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC008798
Gene Symbol C1QL1
Gene ID (NCBI) 10882
RRIDAB_3085471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1QL1 (complement component 1, q subcomponent-like 1) is one of the C1q/tumor-necrosis-factor-related protein (CTRP) family members. C1QL1 is synthesized and secreted mainly by the brain and adipose tissues. It is implicated in synapse formation or elimination in cultured hippocampal neurons in vitro.

实验方案

Product Specific Protocols
WB protocol for C1QL1 antibody 15757-1-APDownload protocol
IHC protocol for C1QL1 antibody 15757-1-APDownload protocol
IF protocol for C1QL1 antibody 15757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols