C1r Polyclonal antibody

C1r Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17346-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

EC:3.4.21.41, Complement C1r subcomponent light chain, Complement C1r subcomponent heavy chain, Complement C1r subcomponent, Complement C1r

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman plasma, human blood
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C1R Ready-To-Use IHC Kit. C1R primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17346-1-AP targets C1r in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human
文献引用反应性human, mouse
免疫原 C1r fusion protein Ag11390 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 1, r subcomponent
别名 EC:3.4.21.41, Complement C1r subcomponent light chain, Complement C1r subcomponent heavy chain, Complement C1r subcomponent, Complement C1r
计算分子量 705 aa, 80 kDa
观测分子量 85 kDa, 30 kDa
GenBank蛋白编号BC035220
基因名称 C1R
Gene ID (NCBI) 715
RRIDAB_2878392
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00736
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation. This antibody recognizes the full-length C1r chain and the cleaved B chain.

实验方案

Product Specific Protocols
WB protocol for C1r antibody 17346-1-APDownload protocol
IHC protocol for C1r antibody 17346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IP,IHC

Mol Carcinog

Downregulation of C1R promotes hepatocellular carcinoma development by activating HIF-1α-regulated glycolysis

Authors - Yuying Ma
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
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