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验证数据展示

  • WB analysis using 24820-1-AP

    WB analysis using 24820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 24820-1-AP

    IF Staining of U2OS using 24820-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C1RL Polyclonal antibody

C1RL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24820-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C1r like protein, C1r LP, C1RL, C1RL1, C1RLP, CLSPa

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24820-1-AP targets C1RL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C1RL fusion protein Ag20324 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 1, r subcomponent-like
Synonyms C1r like protein, C1r LP, C1RL, C1RL1, C1RLP, CLSPa
Calculated Molecular Weight 487 aa, 53 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC156780
Gene Symbol C1RL
Gene ID (NCBI) 51279
RRIDAB_3085758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).

实验方案

Product Specific Protocols
WB protocol for C1RL antibody 24820-1-APDownload protocol
IF protocol for C1RL antibody 24820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols