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C1orf113 Polyclonal antibody

C1orf113 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25767-1-AP

Print datasheet

Synonyms

C1orf113, cDNA FLJ58582, Homo sapiens (Human), SH3D21

验证数据展示

  • IHC staining of mouse testis using 25767-1-AP

    IHC staining of mouse testis using 25767-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25767-1-AP (C1orf113 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25767-1-AP

    IHC staining of mouse testis using 25767-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25767-1-AP (C1orf113 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 25767-1-AP

    WB analysis of mouse testis using 25767-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25767-1-AP (C1orf113 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 25767-1-AP

    IP experiment of mouse testis using 25767-1-AP

    IP result of anti-C1orf113 (IP:25767-1-AP, 4ug; Detection:25767-1-AP 1:500) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25767-1-AP targets C1orf113 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C1orf113 fusion protein Ag22643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 113
Synonyms C1orf113, cDNA FLJ58582, Homo sapiens (Human), SH3D21
Calculated Molecular Weight 640 aa, 71 kDa
Observed Molecular Weight 70-71 kDa
GenBank Accession NumberBC101676
Gene Symbol C1orf113
Gene ID (NCBI) 79729
RRIDAB_2880232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA4FU49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf113 antibody 25767-1-APDownload protocol
IHC protocol for C1orf113 antibody 25767-1-APDownload protocol
IP protocol for C1orf113 antibody 25767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols