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验证数据展示

  • WB analysis using 25361-1-AP

    WB analysis using 25361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25361-1-AP (COA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25361-1-AP

    IHC staining of human colon cancer using 25361-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25361-1-AP (COA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COA7/C1orf163 antibody included
  • IHC staining of human placenta using 25361-1-AP

    IHC staining of human placenta using 25361-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25361-1-AP (COA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COA7/C1orf163 antibody included
  • IF Staining of HeLa using 25361-1-AP

    IF Staining of HeLa using 25361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COA7 antibody (25361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

COA7 Polyclonal antibody

COA7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25361-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

C1orf163, Beta-lactamase hcp-like protein, Sel1 repeat-containing protein 1, Respiratory chain assembly factor 1, RESA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, PC-3 cells
Positive IHC detected inhuman placenta tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy COA7/C1orf163 Ready-To-Use IHC Kit. COA7/C1orf163 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25361-1-AP targets COA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 COA7 fusion protein Ag19734 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 163
别名 C1orf163, Beta-lactamase hcp-like protein, Sel1 repeat-containing protein 1, Respiratory chain assembly factor 1, RESA1
计算分子量 231 aa, 26 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC015313
基因名称 COA7
Gene ID (NCBI) 65260
RRIDAB_2880043
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BR5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c oxidase assembly factor 7 (COA7) is a 231 aa long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins (PMID: 30885959). COA7 is a metazoan-specific assembly factor, the absence or mutation of which in humans accompanies complex IV assembly defects and neurological conditions (PMID: 35210360). COA7 has a molecular weight of ~25 kDa.

实验方案

Product Specific Protocols
WB protocol for COA7 antibody 25361-1-APDownload protocol
IHC protocol for COA7 antibody 25361-1-APDownload protocol
IF protocol for COA7 antibody 25361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Genet

COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.

Authors - Anabel Martinez Lyons
View Article
humanWB

Mol Biol Cell

Discovery of bactericides as an acute mitochondrial membrane damage inducer.

Authors - Ryan Houston
View Article
humanWB,IF

iScience

Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion

Authors - Christina M Knippler
View Article