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COA7 Polyclonal antibody

COA7 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25361-1-AP

Print datasheet

Synonyms

C1orf163, COA7, RESA1, Sel1 repeat-containing protein 1, SELRC1

验证数据展示

  • WB analysis using 25361-1-AP

    WB analysis using 25361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25361-1-AP (COA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 25361-1-AP

    IF Staining of PC-3 using 25361-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 25361-1-AP (C1orf163 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 25361-1-AP

    IHC staining of human colon cancer using 25361-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25361-1-AP (COA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COA7/C1orf163 antibody included
  • IHC staining of human placenta using 25361-1-AP

    IHC staining of human placenta using 25361-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25361-1-AP (COA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COA7/C1orf163 antibody included

经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, PC-3 cells
Positive IHC detected inhuman placenta tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy COA7/C1orf163 Ready-To-Use IHC Kit. COA7/C1orf163 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25361-1-AP targets COA7 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen COA7 fusion protein Ag19734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 163
Synonyms C1orf163, COA7, RESA1, Sel1 repeat-containing protein 1, SELRC1
Calculated Molecular Weight 231 aa, 26 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC015313
Gene Symbol C1orf163
Gene ID (NCBI) 65260
RRIDAB_2880043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BR5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c oxidase assembly factor 7 (COA7) is a 231 aa long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins (PMID: 30885959). COA7 is a metazoan-specific assembly factor, the absence or mutation of which in humans accompanies complex IV assembly defects and neurological conditions (PMID: 35210360). COA7 has a molecular weight of ~25 kDa.

实验方案

Product Specific Protocols
WB protocol for COA7 antibody 25361-1-APDownload protocol
IHC protocol for COA7 antibody 25361-1-APDownload protocol
IF protocol for COA7 antibody 25361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Genet

COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.

Authors - Anabel Martinez Lyons
View Article
humanWB

Mol Biol Cell

Discovery of bactericides as an acute mitochondrial membrane damage inducer.

Authors - Ryan Houston
View Article
humanWB,IF

iScience

Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion

Authors - Christina M Knippler
View Article