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Species Reactivity
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验证数据展示

  • WB analysis using 27969-1-AP

    WB analysis using 27969-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27969-1-AP (C1orf2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

C1orf2 Polyclonal antibody

C1orf2 Polyclonal Antibody for WB, ELISA
Cat No. 27969-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Protein ENTREP3, Protein COTE1, FAM189B, ENTREP3, Endosomal transmembrane epsin interactor 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27969-1-AP targets C1orf2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen C1orf2 fusion protein Ag27428 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 2
Synonyms Protein ENTREP3, Protein COTE1, FAM189B, ENTREP3, Endosomal transmembrane epsin interactor 3
Calculated Molecular Weight 669 aa, 71 kDa
Observed Molecular Weight69-71 kDa
GenBank Accession NumberBC006493
Gene Symbol C1orf2
Gene ID (NCBI) 10712
RRIDAB_3086017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP81408
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf2 is also named COTE1, FAM189B and ENTREP3. C1orf2 is identified as a potential binding partner of a WW domain-containing protein which is involved in apoptosis and tumor suppression. Alternative splicing results in multiple transcript variants. COTE1 contributes to human hepatocarcinogenesis by regulating cell proliferation through the modulation of WWOX signaling, and it upregulated in hepatocellular carcinoma specimens and HCC cell lines (PMID: 23317259, PMID: 24899407).

实验方案

Product Specific Protocols
WB protocol for C1orf2 antibody 27969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols