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  • Featured Product
  • KD/KO Validated

C1orf27 Polyclonal antibody

C1orf27 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22844-1-AP

Print datasheet

Synonyms

C1orf27, hODR 4, LAG1 interacting protein, odr 4, ODR4, Protein odr 4 homolog, TTG1, TTG1A

验证数据展示

  • IHC staining of human breast cancer using 22844-1-AP

    IHC staining of human breast cancer using 22844-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22844-1-AP

    IHC staining of human breast cancer using 22844-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 22844-1-AP

    WB analysis of mouse brain using 22844-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22844-1-AP targets C1orf27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C1orf27 fusion protein Ag18802 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 27
Synonyms C1orf27, hODR 4, LAG1 interacting protein, odr 4, ODR4, Protein odr 4 homolog, TTG1, TTG1A
Calculated Molecular Weight 454 aa, 51 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC003397
Gene Symbol C1orf27
Gene ID (NCBI) 54953
RRIDAB_2879176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SWX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf27 antibody 22844-1-APDownload protocol
IHC protocol for C1orf27 antibody 22844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

J Biol Chem

Human C1orf27 protein interacts with α2A-adrenergic receptor and regulates its anterograde transport.

Authors - Xin Xu
  • KD Validated
View Article