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C1orf35 Polyclonal antibody

C1orf35 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27930-1-AP

Print datasheet

Synonyms

C1orf35, hMMTAG2, MMTAG2

验证数据展示

  • WB analysis using 27930-1-AP

    WB analysis using 27930-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27930-1-AP (C1orf35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27930-1-AP

    IHC staining of human kidney using 27930-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27930-1-AP (C1orf35 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HT-29 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27930-1-AP targets C1orf35 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen C1orf35 fusion protein Ag14374 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 35
Synonyms C1orf35, hMMTAG2, MMTAG2
Calculated Molecular Weight 263 aa, 29 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002843
Gene Symbol C1orf35
Gene ID (NCBI) 79169
RRIDAB_2881011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

27930-1-AP antibody detects the 35 kDa phosphorylated protein in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for C1orf35 antibody 27930-1-APDownload protocol
IHC protocol for C1orf35 antibody 27930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
View Article