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C1orf50 Polyclonal antibody

C1orf50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20957-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HEK-293 using 20957-1-AP

    WB analysis of HEK-293 using 20957-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20957-1-AP (C1orf50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 20957-1-AP

    IHC staining of mouse kidney using 20957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20957-1-AP (C1orf50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 20957-1-AP

    IHC staining of mouse kidney using 20957-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20957-1-AP (C1orf50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, human testis tissue
Positive IHC detected inmouse kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20957-1-AP targets C1orf50 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C1orf50 fusion protein Ag15124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 50
Synonyms
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC001711
Gene Symbol C1orf50
Gene ID (NCBI) 79078
RRIDAB_10733340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf50 antibody 20957-1-APDownload protocol
IHC protocol for C1orf50 antibody 20957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols