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C1orf56 Polyclonal antibody

C1orf56 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25433-1-AP

Print datasheet

Synonyms

AF1Q, C1orf56, MENT, MLLT11, RP11 316M1.9

验证数据展示

  • WB analysis of mouse testis using 25433-1-AP

    WB analysis of mouse testis using 25433-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25433-1-AP (C1orf56 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25433-1-AP

    IHC staining of human liver cancer using 25433-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 25433-1-AP

    IHC staining of human liver cancer using 25433-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25433-1-AP is C1orf56 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C1orf56 fusion protein Ag13860 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 56
Synonyms AF1Q, C1orf56, MENT, MLLT11, RP11 316M1.9
Calculated Molecular Weight 341 aa, 37 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberBC002469
Gene Symbol C1orf56
Gene ID (NCBI) 54964
RRIDAB_2880077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf56, also named as MENT, AF1Q and MLLT11, is involved in control of cellular proliferation. It is extreme expressed in several cancers especially in neuroblastoma. We detected the endogenous protein 35 kDa in mouse testis. We also get a 65 kDa which is unknown.

实验方案

Product Specific Protocols
WB protocol for C1orf56 antibody 25433-1-APDownload protocol
IHC protocol for C1orf56 antibody 25433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.

Authors - Chenghao Zhanghuang
View Article