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C1orf77 Polyclonal antibody

C1orf77 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27478-1-AP

Print datasheet

Synonyms

FOP, CHTOP, PP7704, Friend of PRMT1 protein

验证数据展示

  • WB analysis using 27478-1-AP

    WB analysis using 27478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27478-1-AP (C1orf77 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 27478-1-AP

    IF Staining of A431 using 27478-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (27478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27478-1-AP targets C1orf77 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C1orf77 fusion protein Ag26580 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 77
Synonyms FOP, CHTOP, PP7704, Friend of PRMT1 protein
Calculated Molecular Weight 248 aa, 26 kDa
Observed Molecular Weight26-30 kDa
GenBank Accession NumberBC002733
Gene Symbol C1orf77
Gene ID (NCBI) 26097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3Y2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf77 antibody 27478-1-APDownload protocol
IF protocol for C1orf77 antibody 27478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols