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Complement C2 Polyclonal antibody

Complement C2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27175-1-AP

Print datasheet

Synonyms

C2, complement component 2, Complement C2b fragment, Complement C2a fragment, CO2

验证数据展示

  • WB analysis of A431 using 27175-1-AP

    WB analysis of A431 using 27175-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27175-1-AP

    IHC staining of human liver using 27175-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27175-1-AP

    IHC staining of human liver using 27175-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, Raji cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27175-1-AP targets Complement C2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Complement C2 fusion protein Ag25690 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 2
Synonyms C2, complement component 2, Complement C2b fragment, Complement C2a fragment, CO2
Observed Molecular Weight 70 kDa, 80 kDa
GenBank Accession NumberBC043484
Gene Symbol C2
Gene ID (NCBI) 717
RRIDAB_2880787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Complement C2 antibody 27175-1-APDownload protocol
IHC protocol for Complement C2 antibody 27175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
View Article
IHC

Insect Biochem Mol Biol

Sequence analysis and function of mosquito aeCCC2 and Drosophila Ncc83 orthologs.

Authors - Phu C Duong
View Article