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C20orf195 Polyclonal antibody

C20orf195 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16040-1-AP

Print datasheet

Synonyms

FNDC11, Fibronectin type III domain-containing protein 11

验证数据展示

  • WB analysis of HepG2 using 16040-1-AP

    WB analysis of HepG2 using 16040-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • WB analysis of SMMC-7721 using 16040-1-AP

    WB analysis of SMMC-7721 using 16040-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 16040-1-AP

    IHC staining of human prostate cancer using 16040-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 16040-1-AP

    IHC staining of human prostate cancer using 16040-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16040-1-AP

    IF Staining of HepG2 using 16040-1-AP

    Immunofluorescent analysis of HepG2 cells using 16040-1-AP (C20orf195 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16040-1-AP targets C20orf195 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen C20orf195 fusion protein Ag8914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 20 open reading frame 195
Synonyms FNDC11, Fibronectin type III domain-containing protein 11
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC000912
Gene Symbol C20orf195
Gene ID (NCBI) 79025
RRIDAB_2878209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C20orf195 antibody 16040-1-APDownload protocol
IHC protocol for C20orf195 antibody 16040-1-APDownload protocol
IF protocol for C20orf195 antibody 16040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols