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验证数据展示

  • IHC staining of mouse liver using 31427-1-AP

    IHC staining of mouse liver using 31427-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31427-1-AP (C20orf26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 31427-1-AP

    IF Staining of A431 using 31427-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C20orf26 antibody (31427-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IHC staining of mouse liver using 31427-1-AP

    IHC staining of mouse liver using 31427-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31427-1-AP (C20orf26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C20orf26 Polyclonal antibody

C20orf26 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 31427-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

CFAP61, SPGF84, CaM-IP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31427-1-AP targets C20orf26 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C20orf26 fusion protein Ag35425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 26
别名 CFAP61, SPGF84, CaM-IP3
GenBank蛋白编号BC031674
基因名称 C20orf26
Gene ID (NCBI) 26074
RRIDAB_3669978
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8NHU2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C20orf26, also known as CFAP61 (cilia and flagella associated protein 61), is a protein-coding gene located on human chromosome 20. The protein is predicted to be involved in cilium movement and cilium organization, and it is likely to be located in the axoneme and motile cilium. CFAP61 is also predicted to colocalize with the radial spoke stalk, which is a part of the structural framework of cilia and flagella.

实验方案

Product Specific Protocols
IHC protocol for C20orf26 antibody 31427-1-APDownload protocol
IF protocol for C20orf26 antibody 31427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols