• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

C20orf3/APMAP Polyclonal antibody

C20orf3/APMAP Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25953-1-AP

Print datasheet

Synonyms

C20orf3, UNQ1869/PRO4305, Protein BSCv, BSCv, APMAP

验证数据展示

  • WB analysis of HepG2 using 25953-1-AP

    WB analysis of HepG2 using 25953-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25953-1-AP (C20orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25953-1-AP

    IP experiment of mouse liver using 25953-1-AP

    IP result of anti-C20orf3/APMAP (IP:25953-1-AP, 4ug; Detection:25953-1-AP 1:300) with mouse liver tissue lysate 4800ug.

  • IHC staining of human kidney using 25953-1-AP

    IHC staining of human kidney using 25953-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25953-1-AP (C20orf3 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25953-1-AP targets C20orf3/APMAP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen C20orf3/APMAP fusion protein Ag23029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 20 open reading frame 3
Synonyms C20orf3, UNQ1869/PRO4305, Protein BSCv, BSCv, APMAP
Calculated Molecular Weight 416 aa, 46 kDa
Observed Molecular Weight 42-46 kDa
GenBank Accession NumberBC003501
Gene Symbol APMAP
Gene ID (NCBI) 57136
RRIDAB_2880308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HDC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
IHC protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
IP protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Res

Cholesterol induces epithelial-to-mesenchymal transition of prostate cancer cells by suppressing degradation of EGFR through APMAP.

Authors - Siyuan Jiang
  • KD Validated
View Article
mouseWB

J Nutr Biochem

Thioredoxin domain containing 5 is involved in the hepatic storage of squalene into lipid droplets in a sex-specific way

Authors - Javier Sánchez-Marco
View Article