验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 2 publications below |
产品信息
16633-1-AP targets RTF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | RTF2 fusion protein Ag9972 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromosome 20 open reading frame 43 |
别名 | C20orf43, CDA05, CDAO5, HSPC164, HSPC169, RTF2, SHUJUN 3, UPF0549 protein C20orf43 |
计算分子量 | 34 kDa |
观测分子量 | 35-40 kDa |
GenBank蛋白编号 | BC003359 |
基因名称 | RTF2 |
Gene ID (NCBI) | 51507 |
RRID | AB_2256547 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BY42 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The function of C20orf43 remains largely unknown. Catalog#16633-1-AP is a rabbit polyclonal antibody raised against the full-length of human C20orf43. The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.
实验方案
Product Specific Protocols | |
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WB protocol for RTF2 antibody 16633-1-AP | Download protocol |
IF protocol for RTF2 antibody 16633-1-AP | Download protocol |
IP protocol for RTF2 antibody 16633-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Mol Cell Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
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bioRxiv RTF2 controls replication repriming and ribonucleotide excision at the replisome
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