验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, Y79 cells |
Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23178-1-AP targets MGME1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | MGME1 fusion protein Ag19644 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromosome 20 open reading frame 72 |
别名 | C20orf72, DDK1, MGME1 |
计算分子量 | 344 aa, 39 kDa |
观测分子量 | 36-39 kDa |
GenBank蛋白编号 | BC016869 |
基因名称 | MGME1 |
Gene ID (NCBI) | 92667 |
RRID | AB_2879223 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BQP7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.
实验方案
Product Specific Protocols | |
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WB protocol for MGME1 antibody 23178-1-AP | Download protocol |
IHC protocol for MGME1 antibody 23178-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Immunity Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling.
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Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis
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mBio Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C | ||