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MGME1 Polyclonal antibody

MGME1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23178-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (2)

C20orf72, DDK1, MGME1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, Y79 cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23178-1-AP targets MGME1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 MGME1 fusion protein Ag19644 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 72
别名 C20orf72, DDK1, MGME1
计算分子量 344 aa, 39 kDa
观测分子量 36-39 kDa
GenBank蛋白编号BC016869
基因名称 MGME1
Gene ID (NCBI) 92667
RRIDAB_2879223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQP7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.

实验方案

Product Specific Protocols
WB protocol for MGME1 antibody 23178-1-APDownload protocol
IHC protocol for MGME1 antibody 23178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Immunity

Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling.

Authors - Hongxu Xian
  • KD Validated
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
  • KO Validated
mouseIF,IP

mBio

Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C

Authors - Jianli Tang
humanWB

Mol Cell

The proteomic landscape of genotoxic stress-induced micronuclei

Authors - Kate M MacDonald
humanWB

bioRxiv

Engineering mtDNA Deletions by Reconstituting End-Joining in Human Mitochondria

Authors - Yi Fu
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