验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Hela cells, HepG2 cells, K-562 cells, THP-1 cells, mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32198-1-AP targets C21orf33 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | C21orf33 fusion protein Ag37527 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 21 open reading frame 33 |
| 别名 | GAL3A, Glutamine amidotransferase-like class 1 domain-containing protein 3, mitochondrial, GATD3A, GATD3 |
| 计算分子量 | 268 aa, 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC002370 |
| 基因名称 | C21orf33 |
| Gene ID (NCBI) | 8209 |
| RRID | AB_3670221 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P0DPI2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATD3A is a member of glutamine amidotransferase-like class 1 domain-containing (GATD) family. GATD proteins classically generate ammonia by amidolysis from the glutamine amide nitrogen, transferring it to an acceptor substrate in a variety of anabolic reactions involving purine and pyrimidine synthesis (PMID: 9575335). GATD3A restricts the formation of AGEs in mitochondria and is a relevant target for diseases where AGE deposition is a pathological hallmark (PMID: 35307029).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C21orf33 antibody 32198-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |