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验证数据展示

  • WB analysis of HeLa using 25832-1-AP

    WB analysis of HeLa using 25832-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25832-1-AP (C21orf45 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25832-1-AP

    IF Staining of HepG2 using 25832-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C21orf45 antibody (25832-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

C21orf45 Polyclonal antibody

C21orf45 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25832-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA and More (1)

别名

MIS18A, FASP1, FAPP1-associated protein 1, FAPP1 associated protein 1, C21orf46

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25832-1-AP targets C21orf45 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 C21orf45 fusion protein Ag22922 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 21 open reading frame 45
别名 MIS18A, FASP1, FAPP1-associated protein 1, FAPP1 associated protein 1, C21orf46
计算分子量 233 aa, 26 kDa
观测分子量 105 kDa
GenBank蛋白编号BC042917
基因名称 C21orf45
Gene ID (NCBI) 54069
RRIDAB_2880259
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer(~52 kDa) and heterodimer(~105 kDa) forms.

实验方案

Product Specific Protocols
WB protocol for C21orf45 antibody 25832-1-APDownload protocol
IF protocol for C21orf45 antibody 25832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIF, WB

Br J Pharmacol

Nuclear translocation of ATG5 induces DNA mismatch repair deficiency (MMR-D)/microsatellite instability (MSI) via interacting to Mis18α in colorectal cancer.

Authors - Shi-Yue Sun
View Article