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C21orf59 Polyclonal antibody

C21orf59 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21461-1-AP

Print datasheet

Synonyms

Protein kurly homolog, C21orf48

验证数据展示

  • WB analysis of K-562 using 21461-1-AP

    WB analysis of K-562 using 21461-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 21461-1-AP

    IF Staining of HEK-293 using 21461-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21461-1-AP (C21orf59 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21461-1-AP targets C21orf59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen C21orf59 fusion protein Ag14994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 21 open reading frame 59
Synonyms Protein kurly homolog, C21orf48
Calculated Molecular Weight 290 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000709
Gene Symbol C21orf59
Gene ID (NCBI) 56683
RRIDAB_2878863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57076
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C21orf59 antibody 21461-1-APDownload protocol
IF protocol for C21orf59 antibody 21461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways

Authors - Min Huang
View Article