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  • Featured Product
  • KD/KO Validated

RtcB-Specific Polyclonal antibody

RtcB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19809-1-AP

Print datasheet

Synonyms

HSPC117, RTCB, RNA-splicing ligase RtcB homolog, FAAP, EC:6.5.1.8

验证数据展示

  • WB analysis using 19809-1-AP

    WB analysis using 19809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 19809-1-AP

    IF Staining of HeLa using 19809-1-AP

    Immunofluorescent analysis of HeLa cells using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 19809-1-AP

    IHC staining of human lung cancer using 19809-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RTCB antibody included
  • IP experiment of HeLa using 19809-1-AP

    IP experiment of HeLa using 19809-1-AP

    IP result of anti-RtcB-Specific (IP:19809-1-AP, 4ug; Detection:19809-1-AP 1:4000) with HeLa cells lysate 1800 ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RTCB Ready-To-Use IHC Kit. RTCB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19809-1-AP targets RtcB-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Immunogen RtcB-Specific fusion protein Ag13836 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 22 open reading frame 28
Synonyms HSPC117, RTCB, RNA-splicing ligase RtcB homolog, FAAP, EC:6.5.1.8
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000151
Gene Symbol RTCB
Gene ID (NCBI) 51493
RRIDAB_10695047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

实验方案

Product Specific Protocols
WB protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IHC protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IF protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IP protocol for RtcB-Specific antibody 19809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.

Authors - Yichen Xu
View Article
humanWB,IP

Mol Cell

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.

Authors - Yanyan Lu
View Article
pigWB

Nat Commun

Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.

Authors - Carmen Aguilar
View Article
humanWB

J Biol Chem

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Authors - Irem Unlu
  • KO Validated
View Article
humanIP

Life Sci Alliance

Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.

Authors - Alexandra Papaioannou
View Article
humanWB

J Immunol

The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1

Authors - Lei Cao
View Article